Perez and Barnes were placed on the COVID-19 disabled list on Monday, joining a list of other Red Sox players who will lose time during a critical stretch of the season.
Red Sox manager Alex Cora confirmed the drop in pitchers ahead of Monday’s game in Tampa Bay.
The Red Sox stuck to Major League Baseball’s coronavirus protocols that include contact tracing and diagnostic testing.
Cora reported that Hernández and Arroyo are feeling fine.
Perez is 7-8 with a 4.88 ERA in 31 games this season, with 22 starts.
Barnes has 24 saves along with a 6-5 record in 54 appearances.
The Red Sox, who have struggled most of August, have won three of four heading into the series against the Rays, who ranked first. Boston entered the week eight games behind Tampa Bay in the AL East, but also 2.5 games ahead of the Oakland Athletics for second AL wild-card position.